![]() ![]() The automated measurement of various parameters such as growth, full absorption spectrum, chlorophyll concentration, MALDI-TOF-MS, as well as a novel vitality measurement protocol, have already been established and can be monitored during cultivation. Each microtiter-well within the chamber functions as a separate cultivation vessel with reproducible conditions. The cultivation was performed in 2.2 ml deepwell microtiter plates within a cultivation chamber outfitted with programmable shaking conditions, variable illumination, variable temperature, and an adjustable CO 2 atmosphere. The required technical automation for these processes was achieved with a Tecan Freedom Evo 200 pipetting robot. In this work we describe and test high-throughput methods with the model organism Synechocystis sp. These methods however, have not been generally established for phototrophic organisms such as microalgae or cyanobacteria. High-throughput cultivation and screening methods allow a parallel, miniaturized and cost efficient processing of many samples. ![]()
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